Q: How does this assay work?
A: Our Total Antioxidant Capacity Assay is a colorimetric assay that quantifies antioxidant activity via a SET mechanism and is based on copper reduction. Copper (II)-bathocuproine is used as the chromogenic reagent and the assay limit of detection is 0.0039mM uric acid.
Q: Can this kit be used with samples from any species?
Q: Can this kit be used with samples from any species?
A: Yes, our Total Glutathione Assay is not species specific and should work with any biological protein sample because it measures enzymatic activity rather than protein structure.
Q: Is it possible to test samples that have been stored frozen?
Q: What is the assay principle?
A: The catalase present in the sample breaks down hydrogen peroxide into water and oxygen. The remaining hydrogen peroxide then reacts with ADHP, which produces fluorescence that is measured as an assay read out. This results in a reverse curve, where less catalase activity in the sample results in a higher signal.
Q: Can this kit be used with samples from any species?
A: Yes, our Catalase Assays are not species specific and should work with any biological protein sample because they measure enzymatic activity rather than protein structure.
Q: Is it possible to test samples that have been stored frozen?
Q: Is there an advantage to using the Protein Carbonyl ELISA Kit compared to the fluorometric or spectrophotometric formats?
Q: Why am I getting high background?
Q: Can this kit be used on samples from any species?
A: Because the structure of 3-nitrotyrosine (3-NT) is the same regardless where the protein lysate sample is from, STA-305 is not species specific and can be used for any protein samples containing 3-NT.
Q: What samples types are suitable for this assay?
A: Our AOPP Assay can be used for any samples containing AOPP above the kit detection limit, such as plasma, serum, tissue and cell lysates that have been stored at -80ºC for no more than one year.
Q: What is the sensitivity of this kit?
A: This kit can detect between 49 pg/mL and 200,000 pg/mL of 8-isoprostane, which is the range of the standard curve.