Q: Is this kit compatible with any cell line?
A: Our Cell Transformation Assays, including our In Vitro Tumor Sensitivity Assay, are not cell type specific and can be used for any cell line as long as the cells can form colonies in soft agar.
Q: Can any media be used with this assay?
Q: Which Phagocytosis Assay should I use?
Q: You say manual counting of cells is eliminated when using your Cell Transformation Kits with Cell Recovery, but how can I quantify my cells and still recover them intact for further analysis?
Q: Is it acceptable to add antibiotic to the medium?
A: Antibiotics can be used in the media with this assay, and it will be able to diffuse through the cell agar layer. Any media that is regularly used can be used with this assay, as long as it is 2X.
Q: Does the media need to be replaced during the incubation?
Q: Are your Cell Transformation Assay Kits used the same way as a traditional soft agar assay?
Q: What is the purpose of the Pretreatment Solution?
Q: How is SA-ß-Gal activity measured with this assay?
Q: Is this assay compatible with tissue samples?
A: Yes this kit can be used to stain tissue samples, and no modifications are necessary to the protocol.
Q: I did not detect a blue color with this kit.
Q: Can any of your Cellular Senescence Assays be automated?
Q: Can this assay be used with collagen I coated plates?
A: It is fine to culture cells on any type of plate with the CBA-240 assay. Culturing cells on collagen-I will not affect the assay.
Q: Can this assay be used with bacterial cells?