Q: Can the inserts be reused?
A: Our 24-well migration assays contain 12 individual, removable inserts made out of polycarbonate. Any unused inserts can be stored at 4ºC until needed, however the inserts should not be used more than once.
Q: Can the inserts be stored after staining migratory cells?
Q: How is chemotaxis measured using a Boyden Chamber system?
Q: Does the entire 96-well Invasion Chamber plate need to be used in one experiment?
Q: Is the colorimetric or fluorometric kit more sensitive?
Q: I have never run an assay using a Boyden Chamber. Would I have a better chance of success with your Cell Invasion kits if I use the 24-well or 96-well format?
Q: What size should the tumors be prior to mincing?
A: The size of the tumor used in the assay is not critical because there is a step that adjusts for cell number (step 14 of the Assay Protocol found in the product manual). It is difficult to recommend a size because the cell density will vary, but we used a 160mg lung tumor when developing this assay. If the tumor is large enough to be minced, it will likely have enough cells for the assay.
Q: Is this kit compatible with any cell line?
A: Our Cell Transformation Assays, including our In Vitro Tumor Sensitivity Assay, are not cell type specific and can be used for any cell line as long as the cells can form colonies in soft agar.
Q: Can any media be used with this assay?
Q: Which Phagocytosis Assay should I use?
Q: You say manual counting of cells is eliminated when using your Cell Transformation Kits with Cell Recovery, but how can I quantify my cells and still recover them intact for further analysis?
Q: Is it acceptable to add antibiotic to the medium?
A: Antibiotics can be used in the media with this assay, and it will be able to diffuse through the cell agar layer. Any media that is regularly used can be used with this assay, as long as it is 2X.
Q: Does the media need to be replaced during the incubation?