Q: You say manual counting of cells is eliminated when using your Cell Transformation Kits with Cell Recovery, but how can I quantify my cells and still recover them intact for further analysis?
Q: Is it acceptable to add antibiotic to the medium?
A: Antibiotics can be used in the media with this assay, and it will be able to diffuse through the cell agar layer. Any media that is regularly used can be used with this assay, as long as it is 2X.
Q: Does the media need to be replaced during the incubation?
Q: Are your Cell Transformation Assay Kits used the same way as a traditional soft agar assay?
Q: What is the purpose of the Pretreatment Solution?
Q: How is SA-ß-Gal activity measured with this assay?
Q: Is this assay compatible with tissue samples?
A: Yes this kit can be used to stain tissue samples, and no modifications are necessary to the protocol.
Q: I did not detect a blue color with this kit.
Q: Can any of your Cellular Senescence Assays be automated?
Q: Can this assay be used with collagen I coated plates?
A: It is fine to culture cells on any type of plate with the CBA-240 assay. Culturing cells on collagen-I will not affect the assay.
Q: Can this assay be used with bacterial cells?
Q: Can the plates in your Anoikis Assays be re-used?
Q: How is the gel released from the well?
A: After a two day culture, a sterile spatula is carefully inserted between the plate wall and the gel. The spatula is then slowly moved around the wall in a circle to completely release the gel. We use a small spatula with a thin, flat, edge and is the same spatula used for weighing out chemicals in milligram quantities. Prior to releasing the gel, the spatula should be sterilized with 70% ethanol.