Q: Are your Cell Transformation Assay Kits used the same way as a traditional soft agar assay?

Q: What is the purpose of the Pretreatment Solution?

Q: How is SA-ß-Gal activity measured with this assay?

Q: Is this assay compatible with tissue samples?

A: Yes this kit can be used to stain tissue samples, and no modifications are necessary to the protocol.

Q: I did not detect a blue color with this kit.

Q: Can any of your Cellular Senescence Assays be automated?

Q: Can this assay be used with collagen I coated plates?

A: It is fine to culture cells on any type of plate with the CBA-240 assay.  Culturing cells on collagen-I will not affect the assay.

Q: Can this assay be used with bacterial cells?

Q: Can the plates in your Anoikis Assays be re-used?

Q: How is the gel released from the well?

A: After a two day culture, a sterile spatula is carefully inserted between the plate wall and the gel.  The spatula is then slowly moved around the wall in a circle to completely release the gel.   We use a small spatula with a thin, flat, edge and is the same spatula used for weighing out chemicals in milligram quantities. Prior to releasing the gel, the spatula should be sterilized with 70% ethanol.

Q: How do the Microfluidic Biochips work for studying cell adhesion?

Q: What is the assay principle for your ECM Cell Adhesion Assays?

A: Our Cell Adhesion Assays use an ECM coated plate for cell seeding.  The wells are then washed with PBS to remove any non-adherent cells.  The remaining adherent cells are stained and the dye is then extracted for quantification using a plate reader.