FAQ: Retrovirus Quantitation Kit
Q: How does this kit work?
Q: How does this kit work?
Q: Which expression vector backbone should I use?
Q: What is the difference between the three Platinum cell lines?
A: The Platinum cell lines are used to package retrovirus and they differ in the envelope protein that is expressed. Here is information about each of the Platinum cell lines:
Q: How does this cell line differ from 293T cells?
Q: What is required to make retrovirus?
Q: How does this kit work?
Q: Can this kit be used with any lentivirus pseudotype?
Q: What is the difference between physical titer and infectious titer?
Q: What is required to make lentivirus?
A: To make lentivirus, you need:
Q: How are your 293LTV cells different from HEK293T cells?
A: The 293LTV cell line is a permanent cell line derived from HEK293T and was selected to have a high lentiviral yield. This cell line is fast growing and has a firm attachment to the culture plate, which can sometimes be problematic with standard 293T cells.