Q: Do these cells require culturing on gelatin coated plates?

A: Culturing the SNL cells on gelatin coated plates is optional but it typically is not necessary.

Q: Is it necessary to add LIF to the media?

Q: What is the mouse strain of your MEF cells?

A: Our MEF feeder cells are STO-based cell lines that were isolated from Sandos Inbred Mice (SIM).

Q: Do these cells require culturing on gelatin coated plates?

Q:  Are your Alkaline Phosphatase Kits dependent on species or cell type?

Q: Are the Small GTPase Assay Beads (Raf-RBD, PAK1-PBD and Rhotekin-RBD) species specific?

Q: I am concerned about being able to detect low levels of active small GTPase in my sample using your Small GTPase Activation Assays. Do you have any suggestions to ensure I get good results?

A: There are two things you can do to improve your results with our Small GTPase Activation Assays:

Q: What is the recommended lysis buffer?

Q: How were these cell lines made?

A: Our stable cell lines had GFP, RFP, or luciferase introduced by either lentivirus transduction or plasmid transfection, followed by selection of stable clones.

Q: Can these cell lines be used in vivo?