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The AAV Helper Free System was developed to make production of AAV safer and more convenient. It absolutely eliminates the need for adenovirus transduction! Instead the adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) and in the 293…
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Assay Type
Definition
Cell Types
Pore Size
Insert Coating
Formats
Chemotaxis
Migration of cells toward a chemoattractant (chemical signal) in the cell's surrounding environment
Leukocytes
Neutrophils
3 µm
None
24-…
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Selecting assays for oxidative stress begins with your samples. There are many markers of oxidative stress, but some are more easily detected in certain sample types (cells, tissues, urine, blood, etc.). We have developed a broad portfolio of sensitive, easy-to-use assays to quantify oxidative…
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Use the following table to help choose the right viral vector for your gene expression studies:
Adenovirus
AAV
Lentivirus
Retrovirus
Gene Expression
Transient
Transient
Transient or Stable
Stable
Infect Dividing Cells…
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Plat-A Cells
(Amphotropic)
Plat-E Cells
(Ecotropic)
Plat-GP Cells
(Pantropic*)
Human
+++
N.S.
+++
Mouse
+++
+++
+++
Rat
+++
+++
+++
Monkey
+++
N.S…
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Angiogenesis Assay
Anoikis Assays
Cell Adhesion Assays, ECM
Cell Contraction Assay
Cell Invasion Assays
Cell Migration Assays, 2D Gap Closure
Cell Migration Assays, Chemotaxis
Cell Migration Assays, Haptotaxis
Cell Migration Assays, Transmigration
Cell Transformation…
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Cyclic AMP and GMP Assays
GFP ELISA Kit
GFP Monoclonal Antibody
GFP Quantitation Kit
His-Tag Protein ELISA
Rapid Antibody Purification Kit
Rapid GST Inclusion Body Solubilization and Renaturation Kit
Reporter Stable Cell Lines
RFP Monoclonal Antibody
Rho Kinase (ROCK) Activity Assay, 96-Well
Small…
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General Questions about Oxidative Stress
Antioxidant Assays
Catalase Activity Assay, Colorimetric
Catalase Activity Assay, Fluorometric
Glutathione Assay
HORAC Assay
ORAC Assay
Superoxide Dismutase Activity Assay
Total Antioxidant Capacity (TAC) Assay
DNA / RNA Damage and Repair
8-OHdG ELISA
8…
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Alkaline Phosphatase Assays
MEF Feeder Cells
SNL Feeder Cells
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General Questions about Viral Gene Delivery
AAV (Adeno-Associated Virus) Expression
Using AAV for Gene Delivery
293AAV Cell Line
AAV Expression and Packaging Systems
AAV Purification
AAV Quantitation
AAV Transduction
Adenoviral Expression
Using Adenovirus for Gene Delivery
293AD Cell Line…
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While previous assays only allowed you to detect total ROS and RNS from intact cells, our OxiSelect™ In Vitro ROS/RNS Assay is suitable for use with serum, plasma, urine, cell lysates or cell culture supernatants. This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed…
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Our new Radius™ Cell Migration Assays utilize a proprietary biocompatible hydrogel spotted in the center of each well of a 24-well or 96-well cell culture plate. The gel creates a circular cell-free zone where cells do not attach. Following gel removal, migratory cells move toward the center and…
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AAV-DJ was engineered via DNA family shuffling technology which created a hybrid capsid from 8 AAV serotypes. This resulting vector mediates superior in vitro transduction efficiencies in comparison with any other wild type serotypes and higher infectivity rates across a broad range of cells and…
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Cell migration is a highly integrated, multistep process that plays an important role in the progression of various diseases including cancer, atherosclerosis, arthritis, and mental retardation. To study cell migration in vitro, most researchers have traditionally used a popular tool known as the…
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Oxidative stress is caused by the presence of any of a number of reactive oxygen species (ROS) which the cell is unable to counterbalance. The result is damage to one or more biomolecules including DNA, RNA, proteins and lipids. Oxidative stress has been implicated in the natural aging process as…
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Recombinant viruses are excellent tools for the introduction of genetic material into host cells. There are many choices, and each viral vector has its place. In order to choose the best viral vector for your research goals, ask yourself the following questions:
Do you plan to infect…
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Researchers use viral vectors for gene delivery because of their infectious nature and ability to introduce specific genes into a cell. A virus must first be packaged with the gene of interest to be introduced. The virus is then quantified, purified, and finally transduced into the target cell.…
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Small GTP-binding proteins (GTPases) serve to regulate a variety of cell signaling pathways and are therefore involved in a wide range of cell functions, processes, and morphology. The most studied small GTPase proteins are Ras, Rho, Rac, Cdc42, Rap, and Arf. They regulate molecular events by…
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In the fight against cancer in humans and animals, cancer researchers are the first in a long line of practitioners to take aim at this plague and to ultimately find a cure. Cancer research itself encompasses many specialized areas of study; some researchers focus on potential carcinogens, while…
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Lentivirus vector based on the human immunodeficiency virus-1 (HIV-1) has become a promising vector for gene transfer studies. A popular feature of the lentivirus vector is its ability of gene transfer and integration into both dividing and non-dividing cells. The pseudotyped envelope with…
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Cells go through a natural life cycle which includes growth, maturity, and death. This natural life cycle is regulated by a number of factors, and the disruption of the cycle is involved in many disease states. For example, cancer cells do not die the way normal cells do at the end of their life…
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The most significant consequence of oxidative stress in the body is thought to be damage to DNA. DNA may be modified in a variety of ways, which can ultimately lead to mutations and genomic instability. This could result in the development of a variety of cancers including colon, breast, and…
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In mammals, the process of phagocytosis is essential for a variety of biological events, including tissue remodeling and the continuous clearance of dying cells. Phagocytes (for example macrophages, dendritic cells, and neutrophils) engulf and eliminate foreign invaders such as bacterial or viral…