While previous assays only allowed you to detect total ROS and RNS from intact cells, our OxiSelect™ In Vitro ROS/RNS Assay is suitable for use with serum, plasma, urine, cell lysates or cell culture supernatants. This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed with a dequenching reagent to the highly reactive DCFH form. In the presence of ROS and RNS, the DCFH is rapidly oxidized to the highly fluorescent DCF. For more information, click here.
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