Researchers use viral vectors for gene delivery because of their infectious nature and ability to introduce specific genes into a cell. A virus must first be packaged with the gene of interest to be introduced. The virus is then quantified, purified, and finally transduced into the target cell.
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Recombinant viruses are excellent tools for the introduction of genetic material into host cells. There are many choices, and each viral vector has its place. In order to choose the best viral vector for your research goals, ask yourself the following questions:
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Do you plan to infect dividing or non-dividing cells?
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Do you need transient or stable expression of your gene?
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Are you concerned about a possible immune response from your target cell upon infection?