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Q: What is the detection limit with the CyQuant® dye?
A: The limit of detection with CyQuant® is about 500-1000 cells, which is less sensitive than manual counting. Our recommendation to get the highest level of detection is to seed with the maximum number of cells (500,000) in serum free…
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Q: What is the difference between your product #CBA-101-COL where collagen is pre-coated on the underside (bottom) of the membrane, and your product #CBA 111-COL where collagen is coated on top of the membrane?
A: These products assay for different processes – haptotaxis and cell invasion,…
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Q: What endothelial cells are compatible with this assay?
A: We use HUVEC cells, but any endothelial cells can be used.
Q: How can I confirm that the insert is evenly covered with endothelial cells?
A: After culturing the endothelial cells on the insert for 2-3 days, the insert can be…
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Q: How does the Radius™ assay work?
A: Our Radius assay uses a plate that contains a biocompatible hydrogel at the center of the well; seeded cells will attach everywhere but on the hydrogel. After cells attach, the gel is removed by addition of a removal solution and the assay begins.
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Q: What cell lines are compatible with this assay?
A: Our Wound Healing Assay is compatible with any adherent cell line.
Q: Can the inserts be used with a different plate?
A: We don’t recommend using a different plate with the inserts. The wound inserts are custom made with a plastic…
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Q: I want to test my samples for oxidative stress, but there are so many oxidative stress markers. How do I know which assays to use?
A: Oxidative stress may be tested either directly, by measuring the presence of various reactive oxygen species, or indirectly by measuring the resulting damage…
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Sample preparation
Q: Do I need to extract DNA if using urine, plasma, or serum?
A: It is not necessary to extract DNA if samples are a body fluid such as urine, plasma, serum, cerebrospinal fluid, or saliva. Samples may need to be briefly spun down if there are insoluble particles in the sample,…
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Q: Does this antibody also detect 8-OHdG?
A: The primary antibody used in our 8-OHG kit is monoclonal and will also react with 8-OHdG. We recommend using purified RNA samples to specifically detect 8-OHG. If using urine, serum or cerebrospinal fluid samples, both 8-OHdG and 8-OHG will be…
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Q: Can this be used on samples from any species?
A: The Global DNA Methylation ELISA kit is not species specific because the 5MedCyd modification is the same for all species.
Q: Can I use this on DNA isolated from formalin fixed paraffin embedded tissues?
A: This kit can be used on…
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Q: What damage can be detected with this assay?
A: The Comet assay gives a global picture of DNA damage because it can measure changes caused by double strand breaks, single strand breaks and AP sites. The DNA damage that is able to be detected depends on the running buffer that is selected for…
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Q: Why is 200ng DNA the maximum amount of DNA that can be added?
A: This assay is an indirect ELISA, where the wells are coated with the DNA sample, and the maximum amount of DNA that the well can capture is 200 ng. Adding more than this will not result in an increased signal because the same…
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Q: Are these kits compatible with DNA from paraffin embedded tissues?
A: These kits can be used on any DNA sample, including DNA that has been extracted from paraffin embedded tissues.
Q: Can sheared DNA be used with these assays?
A: The standards are genomic DNA but the DNA size won’…
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Q: Can I use this kit with suspension cells?
A: Our cell-based ELISA kits can be used on suspension cells. The only modification to the protocol necessary will be to spin down the plate before fixing.
Q: Does the CPD-DNA Standard contain only CPD damages or does it also include DNA 6-4PP…
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Q: Is this kit suitable for use with bacterial cells?
A: Since this kit uses isolated DNA samples, it is suitable for use with DNA extracted from bacteria.
Q: Can I use DNA with concentrations lower than 100 µg/ml?
A: It is fine to use samples that are less than 100 µg/ml, as long as the…
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Q: What structures are detected with your AGE Competitive ELISA Kit?
A: Our AGE Competitive ELISA Kit uses an anti-AGE polyclonal antibody that detects multiple AGE structures. The AGE-BSA standard was prepared by reacting BSA with glycolaldehyde, followed by extensive dialysis and column…
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Q: Are EDTA plasma samples compatible with your kit?
A: EDTA will not interfere with our MG ELISA Kit and any anticoagulant can be used when preparing plasma samples.
Q: Is your methylglyoxal kit compatible with samples of any species?
A: Our MG ELISA Kit is not species specific and can…
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Q: What part of the CML does the antibody bind to: the protein or CML itself?
A: The monoclonal antibody used in our CML ELISA Kits recognizes the CML structure, rather than the protein.
Q: Is the antibody in this kit specific to CML or will it recognize other structures?
A: The…
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Q: How does the sensitivity of your MDA ELISA Kit compare to the TBARS Assay?
A: The MDA Adduct Competitive ELISA Kit is more sensitive than the TBARS Assay, but actually the two assays are not directly comparable. The MDA ELISA is more specific and only measures MDA protein adducts, while…
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Q: How long can I store my samples before testing for HNE adducts?
A: Free HNE is very unstable, but HNE adducts are very stable and samples can be stored at -80ºC for up to six months without HNE degradation. Freeze-thaws should be avoided.
Q: Are EDTA plasma samples compatible with…
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Q: Can I use your TBARS Assay to measure the TBARS level in my blood?
A: No, our TBARS Assay Kit is strictly for use by researchers in a laboratory. It cannot be used for diagnostic use to test patient samples.
Q: How does the sensitivity of your MDA ELISA Kits compare to the TBARS Assay…
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Q: What is the sensitivity of this kit?
A: This kit can detect between 49 pg/mL and 200,000 pg/mL of 8-isoprostane, which is the range of the standard curve.
Q: What is considered the normal baseline level of 8-isoprotane in humans?
A: The total normal 8-isoprostane levels are about 40-…
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Q: What samples types are suitable for this assay?
A: Our AOPP Assay can be used for any samples containing AOPP above the kit detection limit, such as plasma, serum, tissue and cell lysates that have been stored at -80ºC for no more than one year.
Q: How do I prepare lysates for the…
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Q: Can this kit be used on samples from any species?
A: Because the structure of 3-nitrotyrosine (3-NT) is the same regardless where the protein lysate sample is from, STA-305 is not species specific and can be used for any protein samples containing 3-NT.
Q: Can I use serum or plasma…
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Q: Why am I getting high background?
A: The high background is likely due to having too much free DNPH remaining on the membrane. DNPH is a sticky molecule and free DNPH can stick to the membrane and be detected by the antibody, causing high background. To avoid background, we recommend…
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Q: Is there an advantage to using the Protein Carbonyl ELISA Kit compared to the fluorometric or spectrophotometric formats?
A: Our Protein Carbonyl ELISA kit is about 5-10 times more sensitive than our Protein Carbonyl Spectrophotometric Assay and only requires 1 µg of protein per assay versus 250…