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Q: How does the sensitivity of your MDA ELISA Kit compare to the TBARS Assay?
A: The MDA Adduct Competitive ELISA Kit is more sensitive than the TBARS Assay, but actually the two assays are not directly comparable. The MDA ELISA is more specific and only measures MDA protein adducts, while…
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Q: How long can I store my samples before testing for HNE adducts?
A: Free HNE is very unstable, but HNE adducts are very stable and samples can be stored at -80ºC for up to six months without HNE degradation. Freeze-thaws should be avoided.
Q: Are EDTA plasma samples compatible with…
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Q: Can I use your TBARS Assay to measure the TBARS level in my blood?
A: No, our TBARS Assay Kit is strictly for use by researchers in a laboratory. It cannot be used for diagnostic use to test patient samples.
Q: How does the sensitivity of your MDA ELISA Kits compare to the TBARS Assay…
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Q: What is the sensitivity of this kit?
A: This kit can detect between 49 pg/mL and 200,000 pg/mL of 8-isoprostane, which is the range of the standard curve.
Q: What is considered the normal baseline level of 8-isoprotane in humans?
A: The total normal 8-isoprostane levels are about 40-…
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Q: What samples types are suitable for this assay?
A: Our AOPP Assay can be used for any samples containing AOPP above the kit detection limit, such as plasma, serum, tissue and cell lysates that have been stored at -80ºC for no more than one year.
Q: How do I prepare lysates for the…
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Q: Can this kit be used on samples from any species?
A: Because the structure of 3-nitrotyrosine (3-NT) is the same regardless where the protein lysate sample is from, STA-305 is not species specific and can be used for any protein samples containing 3-NT.
Q: Can I use serum or plasma…
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Q: Why am I getting high background?
A: The high background is likely due to having too much free DNPH remaining on the membrane. DNPH is a sticky molecule and free DNPH can stick to the membrane and be detected by the antibody, causing high background. To avoid background, we recommend…
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Q: Is there an advantage to using the Protein Carbonyl ELISA Kit compared to the fluorometric or spectrophotometric formats?
A: Our Protein Carbonyl ELISA kit is about 5-10 times more sensitive than our Protein Carbonyl Spectrophotometric Assay and only requires 1 µg of protein per assay versus 250…
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Q: Can this kit be used with samples from any species?
A: Yes, our Catalase Assays are not species specific and should work with any biological protein sample because they measure enzymatic activity rather than protein structure.
Q: Is it possible to test samples that have been stored…
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Q: What is the assay principle?
A: The catalase present in the sample breaks down hydrogen peroxide into water and oxygen. The remaining hydrogen peroxide then reacts with ADHP, which produces fluorescence that is measured as an assay read out. This results in a reverse curve, where less…
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Q: Can this kit be used with samples from any species?
A: Yes, our Total Glutathione Assay is not species specific and should work with any biological protein sample because it measures enzymatic activity rather than protein structure.
Q: Is it possible to test samples that have been stored…
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Q: Can this kit be used with samples from any species?
A: Yes, our SOD Activity Assay is not species specific and should work with any biological protein sample, because it measures enzymatic activity using a xanthine/xanthine oxidase method and does not depend on protein structure which can…
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Q: How does this assay work?
A: Our Total Antioxidant Capacity Assay is a colorimetric assay that quantifies antioxidant activity via a SET mechanism and is based on the ability of an antioxidant to reduce copper(II) ions to copper(I). The assay limit of detection is 0.0039 mM uric acid.
Q: Can…
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Q: Can you please explain the difference between the ORAC, HORAC, and TAC assays and why I would select one over the other?
All three assays measure antioxidant capacity but are slightly different. The ORAC and HORAC assays are both fluorometric assays that use HAT chemistry to detect…
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Q: Can you please explain the difference between the ORAC, HORAC, and TAC assays and why I would select one over the other?
A: All three assays measure antioxidant capacity but are slightly different. The ORAC and HORAC assays are both fluorometric assays that use HAT chemistry to detect…
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Q: What are the differences between the Intracellular ROS Assay and the In Vitro ROS Assay?
A: The main difference between these assays is the sample that is used. Our Intracellular ROS Assay is performed on cultured cells. The cell permeable DCFH-DA is added to cells and is hydrolyzed by…
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Q: What are the differences between the Intracellular ROS Assay and the In Vitro ROS Assay?
A: The main difference between these assays is the sample that is used. Our Intracellular ROS Assay is performed on cultured cells. The cell permeable DCFH-DA is added to cells and is hydrolyzed by…
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Q: Can these kits be used on frozen samples?
A: Both assays #STA-844 and #STA-344 can be used on frozen samples, but due to the transient nature of ROS samples should be frozen for no longer than 1-2 months at -80ºC. The best results will come from fresh samples.
Q: Are cell and tissue samples…
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Q: How do I determine which viral vector to use for gene delivery?
A: There are several considerations when deciding on the best viral vector:
Do I need transient or stable gene expression?
Do I need to infect dividing or non-dividing cells?
How important is potential immune response…
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Q: How should these cells be cultured?
A: The 293AAV cell line was derived from parental HEK293 cells, and the cells were specifically selected to have flattened morphology and firm attachment to improve viral applications. These cells can be cultured the same way you would culture parental 293…
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Q: What are the advantages to using AAV for gene delivery?
A: There are three main advantages to using AAV:
AAV has not been reported to cause any diseases. Together with its replication defective nature, AAV has a good safety profile to be used in gene transfer in vivo and as potential gene…
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Q: What is a bicistronic expression system?
A: A bicistronic vector contains an IRES element that controls the translation of a downstream gene, such as GFP. This means that translation of the GFP mRNA is independent of translation of the gene of interest that is cloned into the MCS, which is…
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Q: Is your ViraDuctin™ AAV Transduction Kit compatible with all cell types?
A: Our ViraDuctin™ AAV Transduction kit works by affecting DNA synthesis, the rate limiting step of viral transduction, and is not cell type or AAV serotype specific. We have not tested it on all cell types; it should…
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Q: Will your AAV Purification kits work with any AAV serotype?
A: Our AAV purification kits use an affinity matrix to purify AAV-2 based on the structure of the AAV2 Cap protein; therefore, they only work for AAV-2 and AAV-DJ and are not suitable for purification of other AAV serotypes. Other…
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Q: Will the QuickTiter™ AAV Quantitation Kit work on all AAV serotypes?
A: This kit can be used on purified AAV of any serotype, including AAV samples that have undergone ultracentrifugation procedures. For crude, unpurified AAV it will only work on AAV serotype 2 and AAV-DJ.
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