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Q: Will this kit detect EGFP?
A: The polyclonal antibodies used in the GFP ELISA kit (AKR-121) are against Jellyfish GFP (Aequorea Victoria) and will detect its variants EGFP, YFP, BFP and CFP, which are only point mutations of wild type GFP. The recombinant GFP standard used in the kit is…
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Q: How does the His-Tag Protein ELISA work?
A: Our His-Tag Protein ELISA (AKR-130) is a competitive ELISA that generates a reverse curve, with the lowest OD values obtained from high His concentrations. First, the sample is added to a plate coated with polyhistidine. The antibody is added…
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Q: What species is this kit compatible with?
A: Our Rapid Antibody Purification Kit is a protein A column based purification kit that can be used to purify IgG from human, mouse, rabbit, pig, dog or cat.
Q: Can I use this kit with more than 5 mL of sample?
A: The capacity of the…
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Q: Will the proteins retrieved with this kit be in their native forms?
A: The proteins retrieved using this kit will be renatured and properly folded, since only correctly folded proteins are able to bind to the glutathione beads.
Q: What is the optimal detergent ratio?
A: Every GST…
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Q: What GFP variants will this antibody recognize?
A: Our anti-GFP antibody is a mouse monoclonal raised against the N-terminal of GFP and will recognize native and denatured GFP and its variants, including EGFP, YFP, EYFP, and CFP.
Q: Does this antibody cross react with dsRed or mCherry…
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Q: Does this antibody cross react with EGFP?
A: Our anti-RFP antibody is a monoclonal antibody raised against the N-terminal peptide sequence of DsRed (VNGHEFEIEGEGEGR). Based on the peptide sequence alignment with EGFP, there is some degree of similarity and there may be some cross reactivity…
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Q: How were these cell lines made?
A: Our stable cell lines had GFP introduced by either lentivirus transduction or plasmid transfection, followed by selection.
Q: Can these cell lines be used in vivo?
A: Although we have not performed any in vivo experiments with these cells, we have many…
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Q: What is the recommended lysis buffer?
A: Cells and tissues can be lysed in RIPA buffer or with the following lysis buffer we have used: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1 mM 2-glycerophosphate, 1 % Triton X-100 or 1 % Nonidet P-40, 1 mM EDTA, 1 mM EGTA, 1 mM Na3VO4 and Proteinase…
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Q: I am concerned about being able to detect low levels of active small GTPase in my sample using your Small GTPase Activation Assays. Do you have any suggestions to ensure I get good results?
A: There are two things you can do to improve your results with our Small GTPase Activation Assays…
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Q: Are the Small GTPase Assay Beads (Raf-RBD, PAK1-PBD and Rhotekin-RBD) species specific?
A: Sequence alignment of a specific small GTPase indicates that there is at most one or two amino acid variation between various species. Therefore our beads and also our primary antibodies may be used…
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Q: Are your Alkaline Phosphatase Kits dependent on species or cell type?
A: All of our Alkaline Phosphatase kits may be used for any cell type that expresses alkaline phosphatase such as osteoblast cells. The kits are not dependent on either cell type or species.
Q: I used your StemTAG…
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Q: What is the mouse strain of your MEF cells?
A: Our MEF feeder cells are STO-based cell lines that were isolated from Sandos Inbred Mice (SIM).
Q: Do these cells require culturing on gelatin coated plates?
A: Culturing the MEF cells on gelatin coated plates is optional but it is…
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Q: Do these cells require culturing on gelatin coated plates?
A: Culturing the SNL cells on gelatin coated plates is optional but it typically is not necessary.
Q: Is it necessary to add LIF to the media?
A: The SNL cell line stably expresses mouse LIF and there is no need to add additional LIF…
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Q: Can this kit be used to detect alcohols other than ethanol?
A: The enzyme used in our Alcohol Assay Kits is not ethanol specific and can be used to detect other alcohols. To use this kit with alcohol samples other than ethanol, the ethanol standard curve should be replaced with your own…
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Q: Are your Cholesterol Assay Kits specific for human samples?
A: Cholesterol is well conserved; therefore our Cholesterol Assay Kits can be used on any mammalian species.
Q: Can EDTA plasma samples be used?
A: Yes, plasma samples prepared with EDTA or any other anticoagulant are…
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Q: Which Oxidized LDL ELISA Kit should I use?
A: Our three OxLDL ELISA kits detect specific modifications on the protein and phospholipid components of LDL. Each uses a different capture antibody for specific modifications (CML, HNE, or MDA) and an anti-apoB antibody for detection. MDA is the most…
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Q: What species is this kit compatible with?
A: The creatinine structure is the same regardless of species; therefore our Urinary Creatinine Assay Kit is not species specific and can be used with urine samples from any species.
Q: Is this kit compatible with serum or plasma?
A: This kit…
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Q: Can this kit distinguish between endothelial and hepatic lipases?
A: Our Lipoprotein Lipase Activity Assay Kit will detect LPL, endothelial, and hepatic lipases, but it is unable to distinguish between the three forms. The LPL fluorometric substrate used in this assay is able to be cleaved…
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Q: What species are your Triglyceride Assay Kits compatible with?
A: Our Triglyceride assays can be used with any mammalian species.
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Alcohol Assays
Cholesterol Assays
Creatinine Assay
Lipoprotein Lipase Activity Assay
Oxidized LDL ELISA Kits
Triglyceride Assays
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MicroRNA, a small non-coding RNA found in animals, plants, and other organisms, plays a big role in our cell processes. But this little molecule wasn't given much consideration until recently. Find out why scientists are putting microRNA in the spotlight of today's research labs.
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A look at the most common forms of damage to our DNA and how that damage can affect our bodies.
Quick Facts about DNA
99.5% of your DNA is shared with every other person on the planet!
The structure of DNA was discovered in 1953 by James Watson and Francis Crick.
DNA’s…