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Cell migration is a highly integrated, multistep process that plays an important role in the progression of various diseases including cancer, atherosclerosis, arthritis, and mental retardation. To study cell migration in vitro, most researchers have traditionally used a popular tool known as the…
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Oxidative stress is caused by the presence of any of a number of reactive oxygen species (ROS) which the cell is unable to counterbalance. The result is damage to one or more biomolecules including DNA, RNA, proteins and lipids. Oxidative stress has been implicated in the natural aging process as…
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Recombinant viruses are excellent tools for the introduction of genetic material into host cells. There are many choices, and each viral vector has its place. In order to choose the best viral vector for your research goals, ask yourself the following questions:
Do you plan to infect…
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Researchers use viral vectors for gene delivery because of their infectious nature and ability to introduce specific genes into a cell. A virus must first be packaged with the gene of interest to be introduced. The virus is then quantified, purified, and finally transduced into the target cell.…
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Small GTP-binding proteins (GTPases) serve to regulate a variety of cell signaling pathways and are therefore involved in a wide range of cell functions, processes, and morphology. The most studied small GTPase proteins are Ras, Rho, Rac, Cdc42, Rap, and Arf. They regulate molecular events by…
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In the fight against cancer in humans and animals, cancer researchers are the first in a long line of practitioners to take aim at this plague and to ultimately find a cure. Cancer research itself encompasses many specialized areas of study; some researchers focus on potential carcinogens, while…
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Lentivirus vector based on the human immunodeficiency virus-1 (HIV-1) has become a promising vector for gene transfer studies. A popular feature of the lentivirus vector is its ability of gene transfer and integration into both dividing and non-dividing cells. The pseudotyped envelope with…
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Cells go through a natural life cycle which includes growth, maturity, and death. This natural life cycle is regulated by a number of factors, and the disruption of the cycle is involved in many disease states. For example, cancer cells do not die the way normal cells do at the end of their life…
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The most significant consequence of oxidative stress in the body is thought to be damage to DNA. DNA may be modified in a variety of ways, which can ultimately lead to mutations and genomic instability. This could result in the development of a variety of cancers including colon, breast, and…
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In mammals, the process of phagocytosis is essential for a variety of biological events, including tissue remodeling and the continuous clearance of dying cells. Phagocytes (for example macrophages, dendritic cells, and neutrophils) engulf and eliminate foreign invaders such as bacterial or viral…
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Neoplastic transformation occurs via a series of genetic and epigenetic alterations that yield a cell population that is capable of proliferating independently of both external and internal signals that normally restrain growth. Anchorage-independent growth is one of the hallmarks of cell…
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Using adenovirus to deliver genes to target cells is an option for researchers who are interested in a high titer virus with high transient expression levels that infects a wide range of cell types. Although adenovirus does not integrate into the genome of the target cell the way a retrovirus…
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Recombinant adeno-associated virus (AAV) is often the preferred method for delivering genes to target cells due to its high titer, mild immune response, ability to infect a broad range of cells, and overall safety. Native AAV can infect humans and primates; however it has not been reported to…
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Q: Can this be used with cells other than HUVECs?
A: This assay can be used with any endothelial cells.
Q: What is the composition of the ECM gel solution?
A: The ECM gel solution is the same as matrigel, which is an ECM extract prepared from Engelbreth-Holm-Swarm (EHS) sarcoma produced in mice…
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Q: What is the assay principle for your ECM Cell Adhesion Assays?
A: Our Cell Adhesion Assays use an ECM coated plate for cell seeding. The wells are then washed with PBS to remove any non-adherent cells. The remaining adherent cells are stained and the dye is then extracted for…
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Q: How do the Microfluidic Biochips work for studying cell adhesion?
A: This product is designed to measure cell adhesion in a microfluidic environment that resembles in vivo shear stresses. The protocol involves coating the biochip with your desired cell adhesion molecule, adding endothelial…
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Q: How is the gel released from the well?
A: After a two day culture, a sterile spatula is carefully inserted between the plate wall and the gel. The spatula is then slowly moved around the wall in a circle to completely release the gel. We use a small sterile spatula with a thin, flat, edge.
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Q: Can the plates in your Anoikis Assays be re-used?
A: Our Anoikis assay uses a hydrogel coated plated that cells cannot attach to, which promotes anoikis cell death. The hydrogel integrity on the surface of the plate may not be maintained throughout the assay protocol and we don’t recommend…
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Q: Can this assay be used with collagen I coated plates?
A: It is fine to culture cells on any type of plate with the CBA-240 assay. Culturing cells on collagen-I will not affect the assay.
Q: Can this assay be used with bacterial cells?
A: Our Cell Viability and Cytotoxicity Assay (…
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Q: Can any of your Cellular Senescence Assays be automated?
A: For automating the senesce assay screening, we recommend using #CBA-231, which comes in a 96-well format and can be read with a fluorescent plate reader. This is an activity assay that quantitates senescence by measuring SA-ß-gal…
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Q: Is this assay compatible with tissue samples?
A: Yes this kit can be used to stain tissue samples, and no modifications are necessary to the protocol.
Q: I did not detect a blue color with this kit.
A: The absence of blue color with the senescence assay indicates that there are few…
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Q: How is SA-ß-Gal activity measured with this assay?
A: This assay uses a fluorometric substrate to detect SA-ß-Gal enzyme activity in cell lysates. The buffers included in this kit maintain pH 6.0 throughout the assay to ensure optimal conditions for measuring SA-ß-Gal while suppressing…
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Q: What is the purpose of the Pretreatment Solution?
A: There are two main types of β-galactosidases in senescent cells: SA-β-galactosidase and Lysosomal β-galactosidase. Lysosomal β-galactosidase activity can only be detected at pH 4. The Cell Pretreatment Solution is used to induce…