Cell Biolabs’ Proline Assay Kit is a simple assay for measuring proline levels in biological samples. Proline levels may be quantified in a wide range of biological samples including serum and plasma. The kit has a detection sensitivity limit of 15.6 µM proline. Each kit provides sufficient reagents to perform up to 100 assays*, including standard curve and unknown samples.
*Note: Each sample replicate requires 2 assays, one treated with Pyrroline-5-carboxylate reductase (+P5CR) and one without (-P5CR). The proline level is calculated from the difference in OD readings at a wavelength of 450 nm from the 2 wells.
