Human Cytomegalovirus Immediate-Early 2 (HCMV IE2) ELISA Kit

HCMV IE2 protein present in the sample or standard binds to the Anti-HCMV IE2 Antibody Coated Plate.  Next, a biotin‑conjugated anti-HCMV IE2 polyclonal antibody is added and binds to the HCMV IE2 protein captured by the first antibody.  Following incubation and wash steps, an HRP-streptavidin conjugate is added and binds to the biotin-conjugated anti-IE2 antibody. Unbound HRP-streptavidin conjugate is removed during a wash step, and a substrate solution reactive with HRP is added to the wells.  A colored product is formed in proportion to the amount of HCMV IE2 protein present in each sample. The reaction is terminated by addition of Stop Solution, and the absorbance is measured at 450 nm.  The HCMV IE2 protein concentration in unknown samples is determined by comparing against a standard curve generated from known concentrations of the protein.