Nitric Oxide Assays

Nitric oxide is a reactive nitrogen species (RNS) which is produced by nitric oxide synthase (NOS). The production of nitric oxide has been established as a mediator in various diseases including diabetes, renal ischemia, cancer, atherosclerosis, and vascular and inflammatory diseases.

We offer two assays for the detection of nitric oxide in a variety of samples:

• Our Intracellular Nitric Oxide Assay uses a fluorescent probe for direct nitric oxide detection in live intact cells. The nitric oxide-specific probe is extremely photostable and provides a wide pH stability range of 5.5 to 9.0.
• Our In Vitro Nitric Oxide Assays measure nitric oxide indirectly via the breakdown products of nitric oxide (nitrite and nitrate) in cell lysates, tissue homogenates, plasma, serum, saliva, urine, and cell culture supernatants. Assays are available for either colorimetric or fluorometric detection in a 96-well plate.