Myeloperoxidase Chlorination Activity Assay

Myeloperoxidase Chlorination Activity Assay, Colorimetric
  • Quantifies MPO chlorination activity from whole neutrophils, neutrophil lysates, tissue homogenates, or EDTA-plasma samples
  • Read results in a standard 96-well colorimetric plate reader
  • Quantify unknown samples against a chromogen standard
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OxiSelect™ Myeloperoxidase Chlorination Activity Assay Kit, Colorimetric
Catalog Number
STA-803
Size
200 Assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$495.00
Product Details

Myeloperoxidase (MPO) is a heme-based peroxidase enzyme responsible for antimicrobial activity against a wide range of organisms. It has also been found to be correlated with increased oxidative stress and decreased levels of catalase activity.

The OxiSelect™ Myeloperoxidase Chlorination Activity Assay Kit is a quantitative colorimetric assay for measuring the myeloperoxidase activity within a sample.  The MPO enzyme catalyzes the reaction of hydrogen peroxide (H2O2) with chloride ions to create hypochlorous acid (HOCl), which rapidly reacts with taurine to produce a stable taurine chloramine product.  This step readily neutralizes the HOCl, which would otherwise accumulate and inactivate MPO.  A catalase-containing stop solution is added to stop MPO catalysis by eliminating hydrogen peroxide.  Finally, taurine chloramine reacts with the yellow TNB chromogen probe, with a decrease in color indicating higher MPO activity.

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  1. Chen, J.L. et al. (2023). Dual phenotypic characteristics of P-selectin in a mouse model of hemorrhagic shock and hepatectomy. Heliyon. 9(8):e18627. doi: 10.1016/j.heliyon.2023.e18627.
  2. Ruiz-Hurtado, P.A. et al. (2021). Evaluation of the gastroprotective effects of Chihuahua propolis on indomethacin- induced gastric ulcers in mouse. Biomed Pharmacother. doi: 10.1016/j.biopha.2021.111345.
  3. Kim, E. et al. (2020). Leucrose, a natural sucrose isomer, suppresses dextran sulfate sodium (DSS)-induced colitis in mice by regulating macrophage polarization via JAK1/STAT6 signaling. J Funct Foods. doi: 10.1016/j.jff.2020.104156.
  4. Cheng, H.S. et al. (2020). Pleiotrosepic ameliorative effects of ellagitannin geraniin against metabolic syndrome induced by high-fat diet in rats. Nutrition. doi: 10.1016/j.nut.2020.110973.
  5. Akman, T. et al. (2020). The ameliorative effect of ozone therapy on spinal cord ischemia in rabbits. Ann Clin Anal Med. 11(3):221-226. doi: 10.4328/ACAM.6215.
  6. Malçok, Ü.A. et al. (2020). Therapeutic effects of syringaldehyde on spinal cord ischemia in rabbits. Saudi Med J. 41(4):341-350. doi: 10.15537/smj.2020.4.24993.
  7. Wang, Q.  et al. (2019). Dysregulated repair and inflammatory responses by e‐cigarette‐derived inhaled nicotine and humectant propylene glycol in a sex‐dependent manner in mouse lung. FASEB BioAdvances. doi:10.1096/fba.2019-00048.
  8. Riyapa, D. et al. (2019). Transketolase and vitamin B1 influence on ROS-dependent neutrophil extracellular traps (NETs) formation. PLoS One. 14(8):e0221016. doi: 10.1371/journal.pone.0221016.
  9. Khan, N.A. et al. (2018). Strain- and sex-dependent pulmonary toxicity of waterpipe smoke in mouse. Physiol Rep. 6(3). doi: 10.14814/phy2.13579.
  10. Sheng Cheng, H. (2017). The Ameliorative Effects of a Tocotrienol-Rich Fraction on the AGE-RAGE Axis and Hypertension in High-Fat-Diet-Fed Rats with Metabolic Syndrome. Nutrients. 9(9): 984. doi: 10.3390/nu9090984.
  11. Goswami, S. K. et al. (2016). Anti-ulcer efficacy of soluble epoxide hydrolase inhibitor TPPU on diclofenac sodium induced intestinal ulcers. J Pharmacol Exp Ther. doi:10.1124/jpet.116.232108.