Lipid Extraction Kit

Lipid Extraction Kit
  • Chloroform-free organic extraction method
  • Extract lipids from plasma, serum, or cultured cells
  • Extraction into upper organic phase is amenable to high-throughput applications
Email To BuyerPrint this PageCopy Link
Ordering

Please contact your distributor for pricing.

Lipid Extraction Kit (Chloroform-Free)
Catalog Number
STA-612
Size
50 preps
Detection
N/A
Manual/Data Sheet Download
SDS Download
Price
$455.00
Product Details

Traditional lipid extraction methods such as the Folch method rely on chloroform, which has two disadvantages: chloroform is a potential carcinogen, and extraction into chloroform results in the lipids being in the lower phase, which is inconvenient.

Our Lipid Extraction Kit eliminates both aforementioned problems by using a chloroform-free organic system that results in the organic phase being on the top. This allows easy removal of the lipid-containing layer as well as adaptation to high-throughput liquid handling systems. Lipids may be extracted from plasma or serum, or from cells grown in culture.

Recent Product Citations
  1. Han, L. et al. (2023). Lipid droplet-associated lncRNA LIPTER preserves cardiac lipid metabolism. Nat Cell Biol. doi: 10.1038/s41556-023-01162-4.
  2. Trombetti, S. et al. (2023). Over-Expressed GATA-1S, the Short Isoform of the Hematopoietic Transcriptional Factor GATA-1, Inhibits Ferroptosis in K562 Myeloid Leukemia Cells by Preventing Lipid Peroxidation. Antioxidants (Basel). 12(3):537. doi: 10.3390/antiox12030537.
  3. Soetikno, V. et al. (2023). Alpha-Mangosteen lessens high-fat/high-glucose diet and low-dose streptozotocin induced-hepatic manifestations in the insulin resistance rat model. Pharm Biol. 61(1):241-248. doi: 10.1080/13880209.2023.2166086.
  4. Dozio, E. et al. (2022). A Wide-Proteome Analysis to Identify Molecular Pathways Involved in Kidney Response to High-Fat Diet in Mice. Int J Mol Sci. 23(7):3809. doi: 10.3390/ijms23073809.
  5. Seki, M. et al. (2022). Local free fatty acids trigger the expression of lipopolysaccharide-binding protein in murine white adipose tissue. Biosci Microbiota Food Health. doi: 10.12938/bmfh.2021-061.
  6. Fukami, H. et al. (2021). Vaccine targeting ANGPTL3 ameliorates dyslipidemia and associated diseases in mouse models of obese dyslipidemia and familial hypercholesterolemia. Cell Rep Med. 2(11):100446. doi: 10.1016/j.xcrm.2021.100446.
  7. Bajaj, A. et al. (2020). Method of extraction and proteome profiling of mycobacteria using liquid chromatography-high resolution mass spectrometry. SN Appl. Sci. doi: 10.1007/s42452-020-03691-1.
  8. Jun, Y. et al. (2020). Leukocyte-Mediated Combined Targeted Chemo and Gene Therapy for Esophageal Cancer. ACS Appl Mater Interfaces. doi: 10.1021/acsami.0c15419.
  9. Greco, C.M. et al. (2020). A non-pharmacological therapeutic approach in the gut triggers distal metabolic rewiring capable of ameliorating diet-induced dysfunctions encompassed by metabolic syndrome. Sci Rep. 10(1):12915. doi: 10.1038/s41598-020-69469-y.
  10. Zhu, Q. et al. (2020). Bupivacaine inhibits angiogenesis through oxidative stress-dependent inhibition of Akt/mTOR and activation of AMPK. Fundam Clin Pharmacol. doi: 10.1111/fcp.12554.
  11. Srisomboon, Y. et al. (2020). Fungal allergen-induced IL-33 secretion involves cholesterol-dependent, VDAC-1-mediated ATP release from the airway epithelium. J Physiol. doi: 10.1113/JP279379.
  12. Srisowanna, N. et al. (2019). The Effect of Estrogen on Hepatic Fat Accumulation during Early Phase of Liver Regeneration after Partial Hepatectomy in Rats. Acta Histochem. Cytochem. 52(4):67-75. doi:10.1267/ahc.19018.
  13. Huang, Z. et al. (2019). ALOX12 inhibition sensitizes breast cancer to chemotherapy via AMPK activation and inhibition of lipid synthesis. Biochem Biophys Res Commun. pii: S0006-291X(19)30743-0. doi: 10.1016/j.bbrc.2019.04.101.
  14. Yang, X. et al. (2018). Inhibiting 6-phosphogluconate dehydrogenase selectively targets breast cancer through AMPK activation. Clin Transl Oncol. 20(9):1145-1152. doi: 10.1007/s12094-018-1833-4.
  15. Matoba, K. et al. (2017). Adipose KLF15 Controls Lipid Handling to Adapt to Nutrient Availability. Cell Rep. 21(11):3129-3140. doi: 10.1016/j.celrep.2017.11.032.
  16. Tyszka-Czochara, M. et al. (2017). Metformin and caffeic acid regulate metabolic reprogramming in human cervical carcinoma SiHa/HTB-35 cells and augment anticancer activity of Cisplatin via cell cycle regulation. Food Chem. Toxicol. 106:260-272.
  17. Maki, T. et al. (2017). Renoprotective effect of a novel selective PPARα modulator K-877 in db/db mice: A role of diacylglycerol-protein kinase C-NAD(P)H oxidase pathway. Metabolism Clinical and Experimental. 71: 33–45.
  18. Tyszka-Czochara, M. et al. (2017). Caffeic Acid Expands Anti-Tumor Effect of Metformin in Human Metastatic Cervical Carcinoma HTB-34 Cells: Implications of AMPK Activation and Impairment of Fatty Acids De Novo Biosynthesis. Int J Mol Sci. doi: 10.3390/ijms18020462.
  19. Pamir, N. et al. (2015). Granulocyte macrophage-colony stimulating factor-dependent dendritic cells restrain lean adipose tissue expansion. J Biol Chem.  doi:10.1074/jbc.M115.645820.