96-Well Leukocyte Endothelium Adhesion Assay

Leukocyte Endothelium Adhesion Assay
  • Quantify interactions between cells and the endothelium
  • Fully quantitative with no manual cell counting
  • Highly sensitive results on a fluorescence plate reader
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CytoSelect™ Leukocyte-endothelium Adhesion Assay
Catalog Number
CBA-210
Size
100 assays
Detection
Fluorometric
Manual/Data Sheet Download
SDS Download
Price
$515.00
Product Details

Leukocyte or tumor cell interactions with vascular endothelium consist of a cascade of processes including the firm attachment of cells to endothelial cell adhesion molecules.

The CytoSelect™ Leukocyte Endothelium Adhesion Assay provides a robust system for the quantitative determination of interactions between leukocytes and the endothelium. Adherent cells can be easily quantified on a fluorescence plate reader.

Assay Principle. Endothelial cells are cultured and form a monolayer, which is treated with desired activator or inhibitor. Meanwhile, leukocytes are labeled with the LeukoTracker™ solution, pelleted, washed and resuspended. Leukocytes are added to the monolayer and incubated. Adherent leukocytes are washed, lysed and quantitated by a fluorometer

Human Monocytic THP-1 Adhesion to HUVEC Monolayer. HUVEC monolayer in a 48-well plate was treated with 1 µM PMA for 12 hours. LeukoTracker™ labeled THP-1 cells were allowed to attach to HUVEC monolayer for 1 hour. Adherent cells were lysed and quantified as described in the assay protocol.

Recent Product Citations
  1. Azúa-López, Z.R. et al. (2023). HDL anti-inflammatory function is impaired and associated with high SAA1 and low APOA4 levels in aneurysmal subarachnoid hemorrhage. J Cereb Blood Flow Metab. doi: 10.1177/0271678X231184806.
  2. Revilla-González, G. et al. (2023). Changes in Adhesion and the Expression of Adhesion Molecules in PBMCs after Aneurysmal Subarachnoid Hemorrhage: Relation to Cerebral Vasospasm. Transl Stroke Res. doi: 10.1007/s12975-023-01136-6.
  3. Zhao, Z. et al. (2021). Gout-induced endothelial impairment: The role of SREBP2 transactivation of YAP. FASEB J. 35(6):e21613. doi: 10.1096/fj.202100337R.
  4. Otto, M. et al. (2021). Apremilast effectively inhibits TNFα-induced vascular inflammation in human endothelial cells. J Eur Acad Dermatol Venereol. doi: 10.1111/jdv.17769.
  5. Bravo, P.L.W. et a. (2021). Antithrombotic Effect of the Ethanol Extract of Angelica gigas Nakai (AGE 232). Life (Basel). 11(9):939. doi: 10.3390/life11090939.
  6. Poursaleh, A. et al. (2021). Adhesion of monocytes and endothelial cells isolated from the human aorta suppresses by miRNA-PEI particles. BMC Cardiovasc Disord. 21(1):395. doi: 10.1186/s12872-021-02203-2.
  7. Gao, X. et al. (2021). Atherogenic oxoaldehyde of cholesterol induces innate immune response in monocytes and macrophages. Cell Biochem Biophys. doi: 10.1007/s12013-021-01004-w.
  8. Johnson, M.D. et al. (2020). CC16 Binding to α4β1 Integrin (VLA-4) Protects Against Mycoplasma pneumoniae Infection. Am J Respir Crit Care Med. doi: 10.1164/rccm.202006-2576OC.
  9. Guo, J. et al. (2020). Hsa-miR-23a-3p promotes atherogenesis in a novel mouse model of atherosclerosis. J Lipid Res. doi: 10.1194/jlr.RA120001121.
  10. Nair, M. et al. (2020). Enhancing Antitumor Efficacy of Heavily Vascularized Tumors by RAMBO Virus through Decreased Tumor Endothelial Cell Activation. Cancers (Basel). 12(4). pii: E1040. doi: 10.3390/cancers12041040.
  11. Sasaki, N. et al. (2020). Rapamycin promotes endothelial-mesenchymal transition during stress-induced premature senescence through the activation of autophagy. Cell Commun Signal. 18(1):43. doi: 10.1186/s12964-020-00533-w.
  12. Liang, J. et al. (2020). Attenuation of pristimerin on TNF-α-induced endothelial inflammation. Int Immunopharmacol. 82:106326. doi: 10.1016/j.intimp.2020.106326.
  13. Abu El-Asrar, A.M. et al. (2019). Galectin-1 studies in proliferative diabetic retinopathy. Acta Ophthalmol. doi: 10.1111/aos.14191.
  14. Li, R. et al. (2018). Attenuation of antimalarial agent hydroxychloroquine on TNF-α-induced endothelial inflammation. Int Immunopharmacol. 63:261-269. doi: 10.1016/j.intimp.2018.08.008.
  15. Hong, B. et al. (2018). Suppression of HMGB1 Released in the Glioblastoma Tumor Microenvironment Reduces Tumoral Edema. Mol Ther Oncolytics. 12:93-102. doi: 10.1016/j.omto.2018.11.005.
  16. Huang, W. et al. (2018). Oridonin inhibits vascular inflammation by blocking NF-κB and MAPK activation. Eur J Pharmacol. 826:133-139. doi: 10.1016/j.ejphar.2018.02.044.
  17. Ramella, M. et al. (2017). Endothelial MMP-9 drives the inflammatory response in abdominal aortic aneurysm (AAA). Am J Transl Res. 9(12):5485-5495.
  18. Wojkowska, D.J. et al. (2017). Interleukin 17A Promotes Lymphocytes Adhesion and Induces CCL2 and CXCL1 Release from Brain Endothelial Cells. Int. J. Mol. Sci. doi: 10.3390/ijms18051000.
  19. Rossignoli, A. et al. (2017). Poliovirus Receptor-Related 2: A Cholesterol-Responsive Gene Affecting Atherosclerosis Development by Modulating Leukocyte Migration. Arterioscler Thromb Vasc Biol. 37(3):534-542. doi: 10.1161/ATVBAHA.116.308715.
  20. He, M. et al. (2016). miR-483 targeting of CTGF suppresses endothelial-to-mesenchymal transition: therapeutic implications in Kawasaki Disease. Circ. Res. doi:10.1161/CIRCRESAHA.116.310233.
  21. Cao, Q. et al. (2016). Myeloid deletion of α1AMPK exacerbates atherosclerosis in LDL receptor knockout (LDLRKO) mice. Diabetes. 65:1565-1576.
  22. Huang, M. et al. (2015). Niclosamide inhibits the inflammatory and angiogenic activation of human umbilical vein endothelial cells. Inflamm Res. 64:1023-1032.
  23. Shah, D. et al. (2015). C1q deficiency promotes pulmonary vascular inflammation and enhances the susceptibility of the lung endothelium to injury. J Biol Chem. doi:10.1074/jbc.M115.690784.
  24. Campos-Estrada, C. et al. (2015). Simvastatin and benznidazole-mediated prevention of Trypanosoma cruzi-induced endothelial activation: role of 15-epi-lipoxin A4 in the action of simvastatin. 9:e0003770.
  25. Ibrahim, A. S. et al. (2015). A lipidomic screen of hyperglycemia-treated HRECs links 12/15-Lipoxygenase to microvascular dysfunction during diabetic retinopathy via NADPH oxidase. J Lipid Res. 56:599-611.
  26. Cao, Q. et al. (2014). Inhibiting DNA Methylation by 5-Aza-2'-deoxycytidine ameliorates atherosclerosis through suppressing macrophage inflammation.Endocrinology. 155:4925-4938.
  27. Cary, L. H. et al. (2014). Interactions between endothelial cells and T cells modulate responses to mixed neutron/gamma radiation. Radiat Res. 181:592-604.
  28. Burrows, G. G. et al. (2014). Human multipotent adult progenitor cells transcriptionally regulate fucosyltransferase VII. Cytotherapy. 16:566-575.
  29. Kapitsinou, P. P. et al. (2014). Endothelial HIF-2 mediates protection and recovery from ischemic kidney injury. J Clin Invest. 124:2396.
  30. Wu, X. Y. et al. (2014). Regulation of microRNA-155 in endothelial inflammation by targeting nuclear factor (NF)-κB p65.  J Cell Biochem. 115:1928-1936.