Hematopoietic stem cells (HSCs) are well-characterized, tissue-specific stem cells that are responsible for the lifelong maintenance of the hematopoietic system. HSCs or hematopoietic progenitors known as colony-forming cells (CFCs) proliferate to form discrete colonies when cultured in a suitable 3D environment, such as methylcellulose supplemented with nutrients and cytokines.
Our CytoSelect™ 96-Well Hematopoietic Colony Forming Cell Assay promotes the formation of HSC colonies in just 7-10 days. Cells can then be either quantified in a fluorescence plate reader or recovered from the CFC assay medium for further downstream analysis.
CytoSelect™ 96-Well Hemtopoietic Colony Forming Cell Assay Principle.
Colony Formation Using the CytoSelect™ 96-Well Hematpoietic Colony Forming Cell Assay.