CPD ELISA for Intact Cells

CPD ELISA for Intact Cells
  • Measure CPD structures of DNA lesions induced by UV light
  • Intact cells are cultured in a 96-well plate and CPD measured by ELISA

 

Frequently Asked Questions about this product

General FAQs about Oxidative Stress

Video: Color Development in an ELISA

Email To BuyerPrint this PageCopy Link
Ordering

Please contact your distributor for pricing.

OxiSelect™ Cellular UV-Induced DNA Damage ELISA Kit (CPD)
Catalog Number
STA-326
Size
96 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$620.00
OxiSelect™ Cellular UV-Induced DNA Damage ELISA Kit (CPD)
Catalog Number
STA-326-5
Size
5 x 96 assays
Detection
Colorimetric
Price
$2,685.00
Product Details

Our OxiSelect™ Cellular UV-Induced DNA Damage ELISA Kit mesaures the formation of cyclobutane pyrimidine dimers (CPD) in intact cells. Cells are first seeded in a 96-well tissue culture plate. Wells are then UV irradiated to induce DNA damage. After fixation and denaturation, cells containing the DNA lesions are probed with an anti-CPD antibody, followed by an HRP conjugated secondary antibody. 

Recent Product Citations
  1. Minoretti, P. et al. (2023) Exploring the Protective Efficacy of Topical Products for Actinic Keratosis Against Ultraviolet-Induced DNA and Protein Damage: An Experimental, Double-Blind Irradiation Study. Cureus. 15(8): e44065. doi: 10.7759/cureus.44065.
  2. Anttila, M.M. et al. (2021). Photoactivatable Reporter to Perform Multiplexed and Temporally Controlled Measurements of Kinase and Protease Activity in Single Cells. Anal Chem. doi: 10.1021/acs.analchem.1c04225.
  3. Espinoza, S. et al. (2019). Characterization of Micro- and Nanoscale LuPO4:Pr3+,Nd3+ with Strong UV-C Emission to Reduce X-Ray Doses in Radiation Therapy. Part. Part. Syst. Charact. doi: 10.1002/ppsc.201900280.
  4. Amirthalingam, M. et al. (2019). Human Mesenchymal Stromal Cells-Derived Conditioned Medium Based Formulation for Advanced Skin Care: in vitro and in vivo Evaluation. J Stem Cell Res Dev Ther. 4: 012. doi: 10.24966/SRDT-2060/100012.
  5. Aditya, A. et al. (2018). ZnO nanoparticles modified with an amphipathic peptide show improved photoprotection in skin. ACS Appl Mater Interfaces. doi: 10.1021/acsami.8b08431.
  6. Guttenplan, J.B. et al. (2017). Effects of Black Raspberry Extract and Berry Compounds on Repair of DNA Damage and Mutagenesis Induced by Chemical and Physical Agents in Human Oral Leukoplakia and Rat Oral Fibroblasts. Chem Res Toxicol. 30(12):2159-2164. doi: 10.1021/acs.chemrestox.7b00242.
  7. Zhang, D. et al. (2017). Echinacoside Alleviates UVB Irradiation-Mediated Skin Damage via Inhibition of Oxidative Stress, DNA Damage, and Apoptosis. Oxid Med Cell Longev. 2017:6851464. doi: 10.1155/2017/6851464
  8. Tang, Q. et al. (2017). Evidence for the role of BmNPV Bm65 protein in the repair of ultraviolet-induced DNA damage. J Invertebr Pathol. 149:82-86. doi: 10.1016/j.jip.2017.08.004.
  9. Dai, W. et al. (2015). A functional single‐nucleotide polymorphism in the ERCC1 gene alters the efficiency of NB‐UVB therapy in active vitiligo patients in a Chinese population. Br J Dermatol. doi: 10.1111/bjd.13892.
  10. Harberts, E. et al. (2015). Ultraviolet radiation signaling through TLR4/MyD88 constrains DNA repair and plays a role in cutaneous immunosuppression. J Immunol.  doi:10.4049/jimmunol.1402583.
  11. Emanuele, E. et al. (2014). Protective effect of trehalose-loaded liposomes against UVB-induced photodamage in human keratinocytes. Biomed Rep. 2:755-759.
  12. Shin, S. et al. (2014). Protective effects of a new phloretin derivative against UVB-induced damage in skin cell model and human volunteers.Int J Mol Sci. 15:18919-18940.
  13. Thongrakard, V. et al. (2013). Protection from UVB toxicity in human keratinocytes by thailand native herbs extracts. Photochem Photobiol. 90:214-224.