Our OxiSelect™ UV-Induced DNA Damage ELISA Combo Kit measures the formation of both CPD and 6-4PP structures in DNA isolated from cells or tissues. Standards or unknown DNA samples are first heat denatured before adsorbed onto a 96-well DNA binding plate. The sample or standard are then probed with an anti-6-4PP or CPD antibody, followed by an HRP conjugated secondary antibody. CPD and 6-4PP structures are assayed on the same plate but in separate wells.
DNA Damage Induced by UV Light. Calf thymus DNA at 0.2 mg/mL was exposed to UV light inside a cell culture hood for the time indicated. The CPD levels in 40 ng denatured DNA samples were determined as described in the Assay Protocol for CPD.
DNA Damage Induced by UV Light. Calf thymus DNA at 0.2 mg/mL was exposed to UV light inside a cell culture hood for the time indicated. The 6-4PP levels in 40 ng denatured DNA samples were determined as described in the Assay Protocol for 6-4PP.
