Cellular Senescence Flow Cytometry Assay

Cellular Senescence Flow Cytometry Assay
  • Uses a fluorogenic substrate to measure senescence-associated ß-galactosidase activity
  • Assay performed in a standard 35 mm culture dish

 

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Quantitative Cellular Senescence Assay (SA β-Gal)
Catalog Number
CBA-232
Size
10 assays
Detection
Fluorescence Microscopy / Flow Cytometry
Manual/Data Sheet Download
SDS Download
Price
$360.00
Quantitative Cellular Senescence Assay (SA β-Gal)
Catalog Number
CBA-232-5
Size
5 x 10 assays
Detection
Fluorescence Microscopy / Flow Cytometry
Price
$1,550.00
Product Details

Our Cellular Senescence Flow Cytometry Assay provides an efficient method to measure Senescence Associated (SA) ß-galactosidase activity. A fluorogenic substrate is added directly to senescent cells in a 35 mm dish. Results can be measured by either flow cytometry or epifluorescence microscope.

Recent Product Citations
  1. Augimeri, G. et al. (2023). A hybrid breast cancer/mesenchymal stem cell population enhances chemoresistance and metastasis. JCI Insight. doi: 10.1172/jci.insight.164216.
  2. Park, S. Y. et al. (2023). Gold nanoparticle encoded with Marigold (Tagetes erecta L.) suppressed hyperglycemia -induced senescence in retinal pigment epithelium via suppression of lipid peroxidation. Arab. J. Chem. doi: 10.1016/j.arabjc.2023.105120.
  3. Viganò, L. et al. (2022). Modulation of the estrogen/erbB2 receptors crosstalk by CDK4/6 inhibition triggers sustained senescence in estrogen receptor and erbB2 positive breast cancer. Clin Cancer Res. doi: 10.1158/1078-0432.CCR-21-3185.
  4. Okawa, R. et al. (2022). The effects of continuous exposure to low-dose chlorine dioxide gas on the characteristics of induced pluripotent stem cells. Regen Ther. 21:250-257. doi: 10.1016/j.reth.2022.07.014.
  5. Cho, E. et al. (2021). Reelin Alleviates Mesenchymal Stem Cell Senescence and Reduces Pathological α-Synuclein Expression in an In Vitro Model of Parkinson’s Disease. Genes. 12(7):1066. doi: 10.3390/genes12071066.
  6. Nishizawa, H. et al. (2021). Lipid peroxidation and the subsequent cell death transmitting from ferroptotic cells to neighboring cells. Cell Death Dis. 12(4):332. doi: 10.1038/s41419-021-03613-y.
  7. Martini, H. et al. (2021). Selective Cardiomyocyte Oxidative Stress Leads to Bystander Senescence of Cardiac Stromal Cells. Int. J. Mol. Sci. 22(5):2245. doi: 10.3390/ijms22052245.
  8. Rothmiller, S. et al. (2021). Chronic senescent human mesenchymal stem cells as possible contributor to the wound healing disorder after exposure to the alkylating agent sulfur mustard. Arch Toxicol. doi: 10.1007/s00204-020-02946-5.
  9. Kim, S.N. et al. (2020). Culturing at Low Cell Density Delays Cellular Senescence of Human Bone Marrow-Derived Mesenchymal Stem Cells in Long-Term Cultures. Int J Stem Cells. doi: 10.15283/ijsc20078.
  10. Jun, E.S. et al. (2020). Gold Nanoparticles Using Ecklonia stolonifera Protect Human Dermal Fibroblasts from UVA-Induced Senescence through Inhibiting MMP-1 and MMP-3. Mar Drugs. 18(9):E433. doi: 10.3390/md18090433.
  11. van den Berg, J. et al. (2020). Development of transient radioresistance during fractionated irradiation in vitro. Radiother Oncol. doi: 10.1016/j.radonc.2020.04.014.
  12. Fessler, J. et al. (2020). Lymphopenia in primary Sjögren's syndrome is associated with premature aging of naïve CD4+ T cells. Rheumatology (Oxford). pii: keaa105. doi: 10.1093/rheumatology/keaa105.
  13. di Martino, S. et al. (2018). HSP90 inhibition alters the chemotherapy-driven rearrangement of the oncogenic secretome. Oncogene. 37(10):1369-1385. doi: 10.1038/s41388-017-0044-8.
  14. Nojima, T. et al. (2018). Deregulated Expression of Mammalian lncRNA through Loss of SPT6 Induces R-Loop Formation, Replication Stress, and Cellular Senescence. Mol Cell. 72(6):970-984.e7. doi: 10.1016/j.molcel.2018.10.011.
  15. Watanabe, J. et al. (2018). Preconditioning of bone marrow-derived mesenchymal stem cells with N-acetyl-L-cysteine enhances bone regeneration via reinforced resistance to oxidative stress. Biomaterials. 185:25-38. doi: 10.1016/j.biomaterials.2018.08.055.
  16. Nagane, M. et al. (2018). Ataxia-Telangiectasia Mutated (ATM) Kinase Regulates eNOS Expression and Modulates Radiosensitivity in Endothelial Cells Exposed to Ionizing Radiation. Radiat Res. 189(5):519-528. doi: 10.1667/RR14781.1.
  17. Poulos, M.G. et al. (2017). Endothelial transplantation rejuvenates aged hematopoietic stem cell function. J Clin Invest. 127(11):4163-4178. doi: 10.1172/JCI93940.
  18. Won, Y.H. et al. (2016). Elucidation of relevant neuroinflammation mechanisms using gene expression profiling in patients with amyotrophic lateral sclerosis. PLoS One 11:e0165290.
  19. Chae, S. Y. et al. (2016). Gardenia jasminoides extract-capped gold nanoparticles reverse hydrogen peroxide-induced premature senescence. J. Photochem Photobiol B.  doi:10.1016/j.jphotobiol.2016.09.033.
  20. Hu, W. et al. (2015). Mechanistic investigation of bone marrow suppression associated with palbociclib and its differentiation from cytotoxic chemotherapies. Clin Cancer Res. doi:10.1158/1078-0432.CCR-15-1421.
  21. Kim, J. et al. (2014) p53 Induces Skin Aging by Depleting Blimp1+ Sebaceous Gland Cells. Cell Death Dis. 5:e1141
  22. Grasso, D. et al. (2014). Genetic Inactivation of the Pancreatitis-Inducible Gene Nupr1 Impairs PanIN Formation by Modulating Kras(G12D)-Induced Senescence. Cell Death Differ. 21:1633-1641.
  23. Landowski, T. H. et al. (2014).  Targeting Integrin α6 Stimulates Curative-Type Bone Metastasis Lesions in a Xenograft Model. Mol Cancer Ther. 13:1558-1566.