Alpha-Ketoglutarate Assay Kits

Alpha-KG Assays
  • 96-well colorimetric or fluorometric detection formats
  • Detect concentrations as low as 4 µM (colorimetric) or 0.2 µM (fluorometric)
  • Suitable for serum, plasma, cell suspensions, and tissue homogenates
  • Alpha-ketoglutarate standard provided for quantitation of unknown samples
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Alpha-Ketoglutarate Assay Kit (Colorimetric)
Catalog Number
MET-5131
Size
100 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$425.00
Alpha-Ketoglutarate Assay Kit (Fluorometric)
Catalog Number
MET-5132
Size
100 assays
Detection
Fluorometric
Manual/Data Sheet Download
SDS Download
Price
$425.00
Product Details

Alpha-ketoglutarate is produced by the oxidative decarboxylation of isocitrate and is also the keto acid product of the deamination of glutamate. It exerts its influence in the TCA cycle as a rate determining intermediary of cellular energy metabolism. It also acts as anitrogen transporter that facilitates amino acid synthesis, while also inhibiting protein degradation in muscles.

Our Alpha-Ketoglutarate Assay Kits provide a simple and convenient method to measure alpha-KG in a 96-well plate format. Alpha-KG is transaminated resulting in the production of pyruvate, which is then detected with either a colorimetric or fluorometric probe. Each kit provides sufficient reagents for 100 assays, including blanks, standards, and unknown samples.

Recent Product Citations

Cappello, A. et al. (2022). Extracellular serine empowers epidermal proliferation and psoriasis-like symptoms. Sci Adv. 8(50):eabm7902. doi: 10.1126/sciadv.abm7902 (#MET-5131).