AAV-5 Packaging System

AAV-5 Packaging System
  • pHelper plasmid contains required E2A, E4, and VA RNA adenoviral genes; eliminates the need for a helper adenovirus
  • Packaging System contains packaging plasmids and GFP control vector; co-transfect with separate AAV expression vector containing your gene of interest

 

Frequently Asked Questions about AAV Expression and Packaging

General FAQs about using AAV

General FAQs about Viral Gene Delivery

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AAV-5 Helper Free Packaging System
Catalog Number
VPK-405
Size
1 kit
Detection
N/A
Manual/Data Sheet Download
SDS Download
Map Download
Sequence Download
Price
$860.00
Product Details

The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

Viral Gene Delivery using the AAV Helper Free System.

Recent Product Citations
  1. Takeda, I. et al. (2022). Controlled activation of cortical astrocytes modulates neuropathic pain-like behaviour. Nat Commun. 13(1):4100. doi: 10.1038/s41467-022-31773-8.
  2. Wang, F. et al. (2022). Thymosin β4 Protects against Cardiac Damage and Subsequent Cardiac Fibrosis in Mice with Myocardial Infarction. Cardiovasc Ther. doi: 10.1155/2022/1308651.
  3. Wu, Y. et al. (2021). BDNF Participates in Chronic Constriction Injury-Induced Neuropathic Pain via Transcriptionally Activating P2X7 in Primary Sensory Neurons. Mol Neurobiol. doi: 10.1007/s12035-021-02410-0.
  4. Nguyen, T.N.T. et al. (2021). Mechanistic Model for Production of Recombinant Adenoassociated Virus via Triple Transfection of HEK293 Cells. Mol Ther Methods Clin Dev. doi: 10.1016/j.omtm.2021.04.006.
  5. Yang, Y., et al. (2020). CREB Participates in Paclitaxel-Induced Neuropathic Pain Genesis Through Transcriptional Activation of Dnmt3a in Primary Sensory Neurons. Neurotherapeutics. doi: 10.1007/s13311-020-00931-5.
  6. Wu, Z. et al. (2020). Gene therapy conversion of striatal astrocytes into GABAergic neurons in mouse models of Huntington's disease. Nat Commun. 11(1):1105. doi: 10.1038/s41467-020-14855-3.
  7. Huang, L.N. et al. (2019). Fn14 Participates in Neuropathic Pain Through NF-κB Pathway in Primary Sensory Neurons. Mol Neurobiol. doi: 10.1007/s12035-019-1545-y.