Leukocyte Transmigration Assay

Leukocyte Transmigration Assay
  • Quantify interactions between endothelium and leukocytes
  • Fully quantify cell transmigration with no manual cell counting
  • Highly sensitive results on a fluorescence plate reader

 

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CytoSelect™ Leukocyte Transmigration Assay
Catalog Number
CBA-212
Size
24 assays
Detection
Fluorometric
Manual/Data Sheet Download
SDS Download
Price
$695.00
Product Details

Cancer cell transmigration, particularly extravasation, is an important step in cancer metastasis. It is the final step in a cascade of interactions between cells and the endothelium.

CytoSelect™ Leukocyte Transmigration Assay provides a robust system for the quantitative determination of transmigrations and interactions between endothelium and leukocytes. Migratory cells may be quantified on a fluorescence plate reader. Kits use 24-well plates with 3 µm pore size membrane inserts.

The Leukocyte Adhesion / Transmigration Cascade.

CytoSelect™ Cell Transmigration Assay Principle.

Recent Product Citations
  1. Zegeye, M.M. et al. (2023). Interleukin-6 trans-signaling induced laminin switch contributes to reduced trans-endothelial migration of granulocytic cells. Atherosclerosis. 371:41-53. doi: 10.1016/j.atherosclerosis.2023.03.010.
  2. Kabbesh, H. et al. (2022). Transmigration of macrophages through primary adult rat Sertoli cells. Tissue Barriers. doi: 10.1080/21688370.2022.2064179.
  3. Sorosina, M. et al. (2022). Involvement of NINJ2 Protein in Inflammation and Blood–Brain Barrier Transmigration of Monocytes in Multiple Sclerosis. Genes. 13(11):1946. doi: 10.3390/genes13111946.
  4. Filiberto, A.C. et al. (2022). Endothelial pannexin-1 channels modulate macrophage and smooth muscle cell activation in abdominal aortic aneurysm formation. Nat Commun. 13(1):1521. doi: 10.1038/s41467-022-29233-4.
  5. Matsumoto, K. et al. (2021). Identification of neutrophil β2-integrin LFA-1 as a potential mechanistic biomarker in ANCA-associated vasculitis via microarray and validation analyses. Arthritis Res Ther. 23(1):136. doi: 10.1186/s13075-021-02510-1.
  6. Shannon, A.H. et al. (2020). Pharmacologic inhibition of transient receptor channel vanilloid 4 attenuates abdominal aortic aneurysm formation. FASEB J. doi: 10.1096/fj.202000251R.
  7. Okumura, K. et al. (2020). Pak1 maintains epidermal stem cells by regulating Langerhans cells and is required for skin carcinogenesis. Oncogene. doi: 10.1038/s41388-020-1323-3.
  8. Hatipoglu, O.F. et al. (2020). Deficiency of CD44 prevents thoracic aortic dissection in a murine model. Sci Rep. 10(1):6869. doi: 10.1038/s41598-020-63824-9.
  9. Stone, M.L. et al. (2017). Mesenchymal stromal cell-derived extracellular vesicles attenuate lung ischemia-reperfusion injury and enhance reconditioning of donor lungs after circulatory death. Respir Res. 18(1):212. doi: 10.1186/s12931-017-0704-9.
  10. Wang, F. et al. (2017). Regulation of Human Brain Microvascular Endothelial Cell Adhesion and Barrier Functions by Memantine. J Mol Neurosci. 62(1):123-129. doi: 10.1007/s12031-017-0917-x.
  11. Choi, J.Y. et al. (2017). KDM4B histone demethylase and G9a regulate expression of vascular adhesion proteins in cerebral microvessels. Sci Rep. 7:45005. doi: 10.1038/srep45005.
  12. Patra, H. K. et al. (2016). Inflammation-sensitive in situ smart scaffolding for regenerative medicine. Nanoscale.  doi:10.1039/C6NR06157E.
  13. White, J. et al. (2016). VLA-4 blockade by natalizumab inhibits sickle reticulocyte and leucocyte adhesion during simulated blood flow. Br J Haematol. doi:10.1111/bjh.14158.
  14. Roberts, L. L.& Robinson, C. M.  (2014). Mycobacterium tuberculosis infection of human dendritic cells decreases integrin expression, adhesion and migration to chemokinesImmunology. 141:39-51.
  15. Giunzioni, I. et al. (2014). Cigarette smoke condensate affects monocyte interaction with endothelium.  Atherosclerosis. 234:383-390.
  16. Roe, K. et al. (2014). West Nile virus-induced cell adhesion molecules on human brain microvascular endothelial cells regulate leukocyte adhesion and modulate permeability of the in vitro blood-brain barrier model. PLoS One. 9:e102598.
  17. Fava, G. et al. (2008). Leptin Enhances Cholangiocarcinoma Cell Growth. Cancer Res. 68:6752-6761.
  18. Zhao, L. et al. (2008). CD44 Expressed on Both Bone Marrow-Derived and Non-Bone Marrow-Derived Cells Promotes Atherogenesis in ApoE-Deficient Mice. Arterioscler. Thromb. Vasc. Biol. 28:1283-1289.