Q: What are the main differences between your two Adenovirus Purification Kits?
A: There are a couple of differences:
1. #VPK-099 uses a spin column while #VPK-5112 uses a purification resin.
2. #VPK-099 can purify one T75 flask or one 10cm dish per prep, while #VPK-5112 can handle up to four flasks or dishes per prep.
Q: Will these kits work with any strain of adenovirus?
A: Our Adenovirus Purification Kits work based on virus surface charge and/or virus size and will work with any strain of adenovirus. Purification of adenoviral lysates results in high virus recovery while removing cellular debris that can interfere with infection.
Q: What is the titer of adenovirus purified with these kits?
A: Our Adenovirus Purification Kits are able to recover at least 60-70% of adenovirus with a similar purity to CsCl preps. The titer obtained with these kits is dependent upon the starting titer, so therefore the titer will vary and there is not one expected titer. We recommend measuring the titer of the crude prep before purification to know the starting titer.
Q: Can I store the virus at -80ºC in elution buffer without adding glycerol?
A: It is fine to store the virus at -80ºC in elution buffer without adding glycerol. Multiple freeze-thaws can kill the virus, so we recommend aliquoting the virus before freezing.