Q: What is the assay principle?
A: The catalase present in the sample breaks down hydrogen peroxide into water and oxygen. The remaining hydrogen peroxide then reacts with ADHP, which produces fluorescence that is measured as an assay read out. This results in a reverse curve, where less catalase activity in the sample results in a higher signal.
Q: Can this kit be used with samples from any species?
A: Yes, our Catalase Assays are not species specific and should work with any biological protein sample because they measure enzymatic activity rather than protein structure.
Q: Is it possible to test samples that have been stored frozen?
A: Two months at -80ºC generally the longest most antioxidant samples should be stored, but we recommend testing fresh samples wherever possible for the best results.
Q: My lysates were prepared in RIPA buffer. Can I use this assay?
A: We have not tested our Fluorometric Catalase Assay with RIPA buffer, but we believe that it may interfere. It is not recommended to use detergents such as SDS on samples that are being tested with an enzymatic assay, because it could affect protein integrity and therefore the enzymatic activity that is being tested. For best results, we recommend preparing lysates by homogenizing or sonicating cells in PBS without the use of detergents.