Q: Is this assay compatible with tissue samples?
A: Yes this kit can be used to stain tissue samples, and no modifications are necessary to the protocol.
Q: I did not detect a blue color with this kit.
A: The absence of blue color with the senescence assay indicates that there are few senescence cells present or that the cells are in the pre-senescence stage. We suggest using a positive control cell line that has a high passage number, such as primary human fibroblasts or WI-38 cells, which are commonly used to study senescence. You may also want to consider using #CBA-231 which is our Cellular Senescence Assay that detects SA-B-Gal activity in a 96-well format. This assay is more sensitive and will detect both senescence and pre-senescence.