5 µm Chemotaxis Assays, 96-Well Format

5 µm Chemotaxis Assays, 96-Well Format
  • Fully quantify chemotaxis with no manual cell counting
  • Measure chemotaxis in less than 6 hours with most cell types
  • Membrane inserts are uncoated to allow use with any chemoattractant
  • Detection with fluorescence plate reader

 

Frequently Asked Questions about this product

General FAQs about Chemotaxis Assays

Email To BuyerPrint this PageCopy Link
Ordering

Please contact your distributor for pricing.

CytoSelect™ 96-Well Cell Migration Assay, 5 µm
Catalog Number
CBA-105
Size
96 assays
Detection
Fluorometric
Manual/Data Sheet Download
SDS Download
Price
$610.00
CytoSelect™ 96-Well Cell Migration Assay, 5 µm
Catalog Number
CBA-105-5
Size
5 x 96 assays
Detection
Fluorometric
Manual/Data Sheet Download
SDS Download
Price
$2,665.00
Product Details

Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their enviroment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases.

CytoSelect™ Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 5 µm pore size is ideal for monocytes / macrophages.

CytoSelect™ Chemotaxis Assay Principle. Migratory cells move through the polycarbonate membrane toward a chemoattractant underneath the membrane inserts.

Recent Product Citations
  1. Emam, A. et al. (2022). Stalled replication fork protection limits cGAS-STING and P-body-dependent innate immune signalling. Nat Cell Biol. 24(7):1154-1164. doi: 10.1038/s41556-022-00950-8.
  2. Ramon-Luing, L.A. et al. (2021). Mycobacterium tuberculosis H37Rv Strain Increases the Frequency of CD3+TCR+ Macrophages and Affects Their Phenotype, but Not Their Migration Ability. Int J Mol Sci. 23(1):329. doi: 10.3390/ijms23010329.
  3. Jin, M. et al. (2021). Tau activates microglia via the PQBP1-cGAS-STING pathway to promote brain inflammation. Nat Commun. 12(1):6565. doi: 10.1038/s41467-021-26851-2.
  4. Nakayama, T. et al. (2020). Type 1 helper T cells generate CXCL9/10-producing T-bet+ effector B cells potentially involved in the pathogenesis of rheumatoid arthritis. Cell Immunol. 360:104263. doi: 10.1016/j.cellimm.2020.104263.
  5. Aota, K. et al. (2020). Inhibition of JAK-STAT Signaling by Baricitinib Reduces Interferon-γ-Induced CXCL10 Production in Human Salivary Gland Ductal Cells. Inflammation. doi: 10.1007/s10753-020-01322-w.
  6. Kumar, S. et al. (2020). Rationalized inhibition of mixed lineage kinase 3 and CD70 enhances life span and antitumor efficacy of CD8+ T cells. J Immunother Cancer. 8(2):e000494. doi: 10.1136/jitc-2019-000494.
  7. Cho, S.Y. et al. (2020). Amplification of transglutaminase 2 enhances tumor-promoting inflammation in gastric cancers. Exp Mol Med. doi: 10.1038/s12276-020-0444-7.
  8. Van Cleemput, J. et al. (2020). An alphaherpesvirus exploits antimicrobial β-defensins to initiate respiratory tract infection. J Virol. pii: JVI.01676-19. doi: 10.1128/JVI.01676-19.
  9. Elsayed, R. et al. (2020). Role of dendritic cell-mediated immune response in oral homeostasis: A new mechanism of osteonecrosis of the jaw. FASEB J. doi: 10.1096/fj.201901819RR.
  10. O'Brien, J.J. et al. (2019). Inhibition of calcium-calmodulin-dependent phosphodiesterase (PDE1) suppresses inflammatory responses. Mol Cell Neurosci. doi: 10.1016/j.mcn.2019.103449.
  11. Chung, K.W. et al. (2019). Mitochondrial Damage and Activation of the STING Pathway Lead to Renal Inflammation and Fibrosis. Cell Metab. pii: S1550-4131(19)30432-2. doi: 10.1016/j.cmet.2019.08.003.
  12. Ouchi, R. et al. (2019). Modeling Steatohepatitis in Humans with Pluripotent Stem Cell-Derived Organoids. Cell Metab. pii: S1550-4131(19)30247-5. doi: 10.1016/j.cmet.2019.05.007.
  13. Denard, B. et al. (2019). Regulating G protein-coupled receptors by topological inversion. Elife. 8. pii: e40234. doi: 10.7554/eLife.40234.
  14. Castro-Sánchez, S. et al. (2019). CX3CR1-deficient microglia shows impaired signalling of the transcription factor NRF2: Implications in tauopathies. Redox Biol. 22:101118. doi: 10.1016/j.redox.2019.101118.
  15. Betto, T. et al. (2019). Vascular endothelial growth factor receptor 1 tyrosine kinase signaling facilitates healing of DSS-induced colitis by accumulation of Tregs in ulcer area. Biomed Pharmacother. 111:131-141. doi: 10.1016/j.biopha.2018.12.021.
  16. Llaudo, I. et al. (2019). C5aR1 regulates migration of suppressive myeloid cells required for costimulatory blockade-induced murine allograft survival. Am J Transplant. 19(3):633-645. doi: 10.1111/ajt.15072.
  17. Kovacsovics, T.J. et al. (2018). Combination of the low anticoagulant heparin CX-01 with chemotherapy for the treatment of acute myeloid leukemia. Blood Adv. 2(4):381-389. doi: 10.1182/bloodadvances.2017013391.
  18. Hakuno, D. et al. (2018). Hepatokine α1-Microglobulin Signaling Exacerbates Inflammation and Disturbs Fibrotic Repair in Mouse Myocardial Infarction. Sci Rep. 8(1):16749. doi: 10.1038/s41598-018-35194-w.
  19. Owens III, A.P. et al. (2018). Tefillin use induces remote ischemic preconditioning pathways in healthy men. Am J Physiol Heart Circ Physiol. 315(6):H1748-H1758. doi: 10.1152/ajpheart.00347.2018.
  20. Mohamed, S.I.A. et al. (2018). Dendritic cells pulsed with generated tumor cell lysate from Phyllanthus amarus Schum. & Thonn. induces anti-tumor immune response. BMC Complement Altern Med. 18(1):232. doi: 10.1186/s12906-018-2296-4.
  21. Yamada, S. et al. (2018). Inhibition of Local Macrophage Growth Ameliorates Focal Inflammation and Suppresses Atherosclerosis. Arterioscler Thromb Vasc Biol. 38(5):994-1006. doi: 10.1161/ATVBAHA.117.310320.
  22. Hernández-Ruiz, E. et al. (2018). The Polycomb proteins RING1B and EZH2 repress the tumoral pro-inflammatory function in metastasizing primary cutaneous squamous cell carcinoma. Carcinogenesis. 39(3):503-513. doi: 10.1093/carcin/bgy016.
  23. Kitadate, A. et al. (2018). Histone deacetylase inhibitors downregulate CCR4 expression and decrease mogamulizumab efficacy in CCR4-positive mature T-cell lymphomas. Haematologica. 103(1):126-135. doi: 10.3324/haematol.2017.177279.
  24. Stabler, T.V. et al. (2017). Chondroitin Sulfate Inhibits Monocyte Chemoattractant Protein-1 Release From 3T3-L1 Adipocytes: A New Treatment Opportunity for Obesity-Related Inflammation? Biomark Insights. 12:1177271917726964. doi: 10.1177/1177271917726964.
  25. Rojas, J.M. et al. (2017). Phospholipase D from Loxosceles laeta Spider Venom Induces IL-6, IL-8, CXCL1/GRO-α, and CCL2/MCP-1 Production in Human Skin Fibroblasts and Stimulates Monocytes Migration. Toxins (Basel). 9(4). pii: E125. doi: 10.3390/toxins9040125.
  26. Luo, X. et al. (2017). Methylation-mediated loss of SFRP2 enhances melanoma cell invasion via Wnt signaling. Am J Transl Res. 8(3):1502-9.
  27. Shikhagaie, M.M. et al. (2017). Neuropilin-1 Is Expressed on Lymphoid Tissue Residing LTi-like Group 3 Innate Lymphoid Cells and Associated with Ectopic Lymphoid Aggregates. Cell Rep. doi: 10.1016/j.celrep.2017.01.063.
  28. Vazquez Rodriguez, G. et al. (2017). Estradiol Promotes Breast Cancer Cell Migration via Recruitment and Activation of Neutrophils. Cancer Immunol Res. doi: 10.1158/2326-6066.CIR-16-0150.
  29. Cao, Q. et al. (2016). Myeloid deletion of α1AMPK exacerbates atherosclerosis in LDL receptor knockout (LDLRKO) mice. Diabetes. 65:1565-1576.
  30. Yao, L. et al. (2016). Characterization of liver monocytic myeloid-derived suppressor cells and their role in a murine model of non-alcoholic fatty liver disease. PLoS One. 11:e0149948.